Figure 6.

RNF168 induced prolonged ubiquitination at DDR foci. A) and B) HeLa cells transfected with GFP-RNF168, GFP-RF* or the vector alone were treated with etoposide (5 μM) for 1 hr and then either fixed (A) or chased for additional 7 hours (B). IF analysis was performed with anti-Ub antibodies (FK2).