Research article
RNF168, a new RING finger, MIU-containing protein that modifies chromatin by ubiquitination of histones H2A and H2AX
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* Corresponding author: Lorenza Penengo lorenza.penengo@pharm.unipmn.it
- † Equal contributors
1 Department of DISCAFF and DFB Center, University of Piemonte Orientale "A. Avogadro", Novara, Italy
2 Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands
BMC Molecular Biology 2009, 10:55 doi:10.1186/1471-2199-10-55
Published: 5 June 2009Additional files
Additional file 1:
Additional Figure 1 RNF168 Ub-ligase activity. HeLa cells transfected with cDNA encoding the GFP-tagged version of RNF168 and its RF-defective mutant (RF*) were immunostained using antibodies that recognize ubiquitinated proteins (FK2).
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Additional file 2:
Additional Figure 2 RNF168 ubiquitinates histone H2A in vivo. Cells transfected with the indicated GFP-RNF168, GFP-RF* or the vector alone were immunostained with anti-uH2A.
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