Gel mobility shift assay of the promoter DNA of PP1636. Approximately 0.5 ng (1000 cpm) of the DNA probe was incubated with different amounts of histidine-tagged purified ColR (lanes 2 to 4). To test the effect of phosphorylation on ColR binding activity, ColS and ATP were added to the reactions (lanes 5 to 7).
Kivistik et al. BMC Molecular Biology 2009 10:46 doi:10.1186/1471-2199-10-46