Table 1

Strains and plasmids used or generated in this study.

Name

Main characteristics/accession number

Source or reference


Strains

Pantoea ananatis SC17 (AJ13355)

mutant with decreased secretion of mucus

[1]

Pantoea ananatis SC17(0)

Derivative of SC17 resistant to expression of λ Red genes

This work

E. coli K12 MG1655

Wild type

VKPM


Plasmids

RSF1010

GenBank accession number NC_001740

[26]

pUC4K

GenBank accession number X06404

[32]

pMW-attLλ-KmR-attRλ

Donor attLλ-KmR-attRλ cassette; ApR; KmR

[35]

pKD46

pINT-ts; λ gam, bet, and exo genes are under ParaB promoter; ApR

[4]

pRSFRedTER

λ gam, bet, and exo genes are under control of P-element; sacB gene; CmR

This work

pRSFRedkan

λ gam, bet, and exo genes are under control of P-element; KmR

This work

pRSFGamBet

λ gam and bet genes are under control of P- element; sacB gene; CmR

This work

pRSFGamBetkan

λ gam and bet genes are under control of P- element; KmR

This work

pRSFPlacsacB

PlacUV5-sacB-cat cassette; CmR

This work

pMW-intxis-cat

pSC101-ts; λ xis-int genes transcribed from λ PR promoter under CIts857 control; CmR

This work


A P-element marks the auto-regulated element PlacUV5-lacI [see [31] for details].

Katashkina et al. BMC Molecular Biology 2009 10:34   doi:10.1186/1471-2199-10-34

Open Data