Table 1 |
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Strains and plasmids used or generated in this study. |
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|
Name |
Main characteristics/accession number |
Source or reference |
|
|
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|
Strains |
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|
Pantoea ananatis SC17 (AJ13355) |
mutant with decreased secretion of mucus |
[1] |
|
Pantoea ananatis SC17(0) |
Derivative of SC17 resistant to expression of λ Red genes |
This work |
|
E. coli K12 MG1655 |
Wild type |
VKPM |
|
|
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|
Plasmids |
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|
RSF1010 |
GenBank accession number NC_001740 |
[26] |
|
pUC4K |
GenBank accession number X06404 |
[32] |
|
pMW-attLλ-KmR-attRλ |
Donor attLλ-KmR-attRλ cassette; ApR; KmR |
[35] |
|
pKD46 |
pINT-ts; λ gam, bet, and exo genes are under ParaB promoter; ApR |
[4] |
|
pRSFRedTER |
λ gam, bet, and exo genes are under control of P-element; sacB gene; CmR |
This work |
|
pRSFRedkan |
λ gam, bet, and exo genes are under control of P-element; KmR |
This work |
|
pRSFGamBet |
λ gam and bet genes are under control of P- element; sacB gene; CmR |
This work |
|
pRSFGamBetkan |
λ gam and bet genes are under control of P- element; KmR |
This work |
|
pRSFPlacsacB |
PlacUV5-sacB-cat cassette; CmR |
This work |
|
pMW-intxis-cat |
pSC101-ts; λ xis-int genes transcribed from λ PR promoter under CIts857 control; CmR |
This work |
|
|
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|
A P-element marks the auto-regulated element PlacUV5-lacI [see [31] for details]. |
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|
Katashkina et al. BMC Molecular Biology 2009 10:34 doi:10.1186/1471-2199-10-34 |
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