Analysis of mRNA integrity. Comparison of FASN (a) and GAPDH (b) mRNA 5'/3' ratios in placental samples stored at + 4°C for 0 to 96 h, as determined by qRT-PCR assays targeting sequences close to the 5' and 3' ends of the transcripts. RNA samples prepared with protocol A were compared to protocol B (n = 14 in each group). Results are expressed as mean +/- SEM. Each experiment was performed in duplicate. p values were determined by ANOVA. p < 0.05 was considered to be significant. FASN 5'/3', protocol A versus protocol B (p = 0.04), at any time of storage.
Fajardy et al. BMC Molecular Biology 2009 10:21 doi:10.1186/1471-2199-10-21