Analysis of total RNA integrity according to delay time of storage and to handling conditions. The integrity of total RNA in placental tissues stored at + 4°C for 0 to 96 H, was determined by Agilent Bioanalyzer assay. Results (mean +/- SEM, each experiment in duplicate) were expressed as RIN values (top of the figure), or 28S:18S ratios (bottom of the figure). RNA was prepared from placental samples transferred directly to RNA later™(protocol A), or dissected before banking (protocol B). p values were determined by ANOVA. p < 0.05 was considered to be significant. RIN protocol A versus Protocol B: p = 0.007.
Fajardy et al. BMC Molecular Biology 2009 10:21 doi:10.1186/1471-2199-10-21