Open Access Highly Accessed Methodology article

Selection of suitable housekeeping genes for expression analysis in glioblastoma using quantitative RT-PCR

Valeria Valente12*, Silvia A Teixeira1, Luciano Neder3, Oswaldo K Okamoto4, Sueli M Oba-Shinjo5, Suely KN Marie5, Carlos A Scrideli6, Maria L Paçó-Larson2 and Carlos G Carlotti1

Author Affiliations

1 Department of Surgery and Anatomy, Faculty of Medicine, University of São Paulo, Av. dos Bandeirantes 3900, 140490-900, Ribeirão Preto, SP, Brazil

2 Department of Cellular and Molecular Biology, Faculty of Medicine, University of São Paulo, Av. dos Bandeirantes 3900, 140490-900, Ribeirão Preto, SP, Brazil

3 Department of Pathology, Faculty of Medicine, University of São Paulo, Av. dos Bandeirantes 3900, 140490-900, Ribeirão Preto, SP, Brazil

4 Department of Neurology and Neurosurgery, Federal University of São Paulo, R. Botucatu 740, 04023-900, São Paulo, SP, Brazil

5 Department of Neurology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 01246903, São Paulo, SP, Brazil

6 Department of Pediatrics, Faculty of Medicine, University of São Paulo, Av. dos Bandeirantes 3900, 140490-900, Ribeirão Preto, SP, Brazil

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BMC Molecular Biology 2009, 10:17  doi:10.1186/1471-2199-10-17

Published: 3 March 2009

Additional files

Additional file 1:

Expression levels of target genes in normal and tumor tissues upon different normalization approaches. Median relative quantities of the indicated target genes in non-neoplastic white matter (black bars) and glioblastoma (gray bars) samples after normalization with: geNorm normalization factors calculated from TBP plus HPRT1 and with the genes TBP or HPRT1 alone. Asterisks indicate the significance of differences, * P values < 0.05 and ** P values < 0,005.

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