Figure 9.

The presence of CPEB3 in P60 mouse retina. We used double-immunofluorescence labeling with antibodies to CPEB3 and ChAT. ChAT is a marker for cholinergic amacrine cells. A few cells in the RGC layer were double-labeled with ChAT and CPEB3 antibodies, as indicated with the arrows. This suggested that a few CPEB3 positive cells in the RGC layer were displaced amacrine cells. The size of such cells was usually smaller than those cells staining positive for both CPEB3 and Map1a. The INL was also labeled with CPEB3, with the innermost cells more intensely labeled. A few cells in the INL appeared to be double-immunopositive for ChAT and CPEB3 (arrowhead), suggesting that some CPEB3 positive cells in the INL were cholinergic amacrine cells. Both the IPL and the OPL were labeled with CPEB3. Scale bar represented 50 μm.