Research article
Quantitative TaqMan® real-time PCR assays for gene expression normalisation in feline tissues
-
* Corresponding author: Regina Hofmann-Lehmann rhofmann@vetclinics.uzh.ch
- † Equal contributors
1 Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland
2 Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Switzerland
3 Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Switzerland
BMC Molecular Biology 2009, 10:106 doi:10.1186/1471-2199-10-106
Published: 11 December 2009Additional files
Additional file 1:
NormFinder output. Stability values and standard errors calculated by the NormFinder program. In addition, a gene ranking for every tissue is shown.
Format: PDF Size: 160KB Download file
This file can be viewed with: Adobe Acrobat Reader
Additional file 2:
geNorm output files. Average expression stability values calculated by the geNorm program for reference genes included in this study and graph showing the optimal number of reference genes for normalization. Output files are shown for a) adrenal gland, b) pancreas, c) parathyroid, d) thyroid, e) bone marrow, f) lymph node, g) spleen, h) parotid gland, i) duodenum, j) ileum, k) liver, l) kidney, m) myocardium, n) brain, o) blood, p) neoplastic tissues, q) all 14 healthy tissues combined, r) all endocrine tissues, s) all lymphoid tissues, and t) all gastrointestinal tissues.
Format: PDF Size: 634KB Download file
This file can be viewed with: Adobe Acrobat Reader