Research article
piggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome
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* Corresponding author: John H Adams jadams3@health.usf.edu
1 Department of Global Health, 3720 Spectrum Blvd Suite 304, University of South Florida, Tampa, Florida 33612, USA
2 Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA
3 Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia 30602, USA
4 Department of Genetics, University of Georgia, Athens, Georgia 30602, USA
BMC Microbiology 2009, 9:83 doi:10.1186/1471-2180-9-83
Published: 7 May 2009Additional files
Additional file 1:
List of piggyBac insertion loci in the P. falciparum genome. Complete list of piggyBac insertion loci identified thus far is provided along with the mutant name and insertion position relative to the coding sequences of the genome.
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Additional file 2:
Best-fit growth curve models for doubling time estimation of mutant clones. The predicted best-fit and observed growth curves for each parasite clone is shown.
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Additional file 3:
Lack of gene expression in mutant P. falciparum clones with insertions in the coding sequences. RT-PCR analysis confirms the knockout of gene expression in mutant clones, selected for growth assays, with insertions in coding sequences.
Format: PDF Size: 157KB Download file
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