Open Access Highly Accessed Research article

Staphylococcus epidermidis strains isolated from breast milk of women suffering infectious mastitis: potential virulence traits and resistance to antibiotics

Susana Delgado1, Rebeca Arroyo1, Esther Jiménez1, Maria L Marín1, Rosa del Campo2, Leonides Fernández1 and Juan M Rodríguez1*

Author Affiliations

1 Dpt. Nutrición, Bromatología y Tecnología de los Alimentos. Universidad Complutense de Madrid, 28040 Madrid, Spain

2 Servicio de Microbiología, Hospital Ramón y Cajal, 28034 Madrid, Spain

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BMC Microbiology 2009, 9:82  doi:10.1186/1471-2180-9-82

Published: 7 May 2009

Additional files

Additional file 1:

PCR-RFLP of the ccrB gene using endonucleases HinfI and HinfI/BsmI. The figure provided shows the profiles of SCCmec types III and IV using the method of Yang et al. [40]. In lanes 1 and 3 ccrB amplicons are cut with HinfI whereas in lanes 2 and 4 the amplicons are cut with HinfI and BsmI. Lanes 1 and 2: S. epidermidis DF2LAB, SCCmec type III (537, 106 bp and 320, 174, 106 bp respectively); lanes 3 and 4: S. epidermidis V1LD1, SCCmec type IV (264, 227, 154 and 227, 171, 153, 93 bp respectively); M, molecular weight marker.

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Additional file 2:

Multiplex tuf gene-based PCR assay for the specific identification of S. aureus and S. epidermidis. The figure provided shows the respective species-specific bands. Lanes: 1–5, S. aureus isolates; 6–9, S. epidermidis isolates, 10, negative control; M, molecular weight marker (100 bp Ladder, Invitrogen).

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Additional file 3:

Multiplex PCR assay for the simultaneous detection of three adhesion- or biofilm-related genes. The figure provided shows the respective gene-specific bands. Lanes: 1, S. epidermidis CJBP2; 2, S. epidermidis V1LD1; 3, S. epidermidis DG2S; 4, S. epidermidis P2LD1; 5, S. epidermidis S1LDC13; 6, negative control; M, molecular weight marker. atlE gene: 682 bp; fbe gene: 496 bp; icaD gene: 225 bp.

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