Immunofluorescence double staining of ADAM9 and HPIV2 marker of HPIV2 stimulated GMK cell cultures on culture day 0 (panel A, B), 1 (panel C), 3 (panel D). ADAM9 staining is shown in red and HPIV2 shown in green, together with the blue nuclear counterstain of the same field. On culture day 0 HPIV2 did not infect any cells, therefore (A) shows only nuclear counterstain, (B) shows only ADAM9 staining, (C, D) overlays show double staining of ADAM9 and HPIV2 marker on culture days 1 and 3, respectively. Bar = 10 μm.
Ma et al. BMC Microbiology 2009 9:55 doi:10.1186/1471-2180-9-55