Table 4

Summary of Igl1 protein levels in Igl shRNA transfectants

shRNA Transfectant or Control Sample

% of Igl1 protein level (± SE)

P-value


GFP

100.0 ± 3.6

HM1:IMSS

115.5 ± 11.8

0.1449

Igl (2412–2440)

95.3 ± 3.2

0.2078

Igl1 (272–300)

27.8 ± 1.3

< 0.0001

Igl (1198–1226)

42.3 ± 2.1

< 0.0001


Igl (2777–2805)

38.1 ± 3.1

< 0.0001


The average level of Igl1 protein in the GFP control shRNA transfectants was defined as 100% expression of Igl1 protein for computational purposes. Protein levels of Igl1, using actin as a standard for comparison, were quantified from Western blotting. Values are expressed as the percentage of the GFP level ± SE, with the P-value following each, calculated using Student's t test. For Western blotting, there were three biological replicates, each run in triplicate sets of serial dilutions (1:2, 1:4, and 1:8), with the exception of the HM1:IMSS nontransfected samples having one biological replicate rather than three. Protein levels were not statistically different between the Igl1 (272–300), Igl (1198–1226), and Igl (2777–2805) samples (tested with ANOVA, one-tailed, α = 0.05, 0.1 < P < 0.25) or the GFP, HM1:IMSS, and Igl (2412–2440) samples (tested with ANOVA, one-tailed, α = 0.05, P > 0.25). A representative Western blot is shown in Figure 2.

Linford et al. BMC Microbiology 2009 9:38   doi:10.1186/1471-2180-9-38

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