Table 4

Plasmids used in this work.

Plasmids

Characteristics

Reference


pIV2

cloning vector with an origin of replication functional in Enterobacteriacea and a kanamycin resistance gene

[39]


pLitmus38

cloning vector with the origin of replication from pUC, an ampicillin resitance gene and the lacZ' gene for blue/white selection

New England Biolabs


pMV306

cloning vector replicating in E. coli with the kanamycin resistance gene aph from transposon Tn903 and the gene for the integrase and the attP site of phage L5 for integration into the mycobacterial genome

[40]


pMV261

Mycobacterium/E. coli shuttle vector with the kanamycin resistance gene aph from transposon Tn903 and the promoter from the hsp60 gene from M. tuberculosis

[40]


pSHKLx1

Mycobacterium/E. coli shuttle vector with a kanamycin resistance gene from Tn5, a hygromycin resistance gene and the promoter from the hsp60 gene from M. tuberculosis

[43]


pSSa100

pMV306 with a 3429 bp genomic DNA fragment from M. smegmatis SMR5 carrying mspA

[13]


pSSp107, pSSp108

pIV2 with a 2895 bp genomic DNA fragment from M. fortuitum 10860/03 carrying the porM1 gene

This study


pSRb101

pMV261 carrying the porM1 gene from M. fortuitum 10860/03

This study


pSRb103

pMV261 carrying the porM2 gene from M. fortuitum 10851/03

This study


pSRa102

pMV306 carrying the porM1 gene from M. fortuitum 10860/03

This study


pSRa104

pMV306 carrying the porM2 gene from M. fortuitum 10851/03

This study


pSRr106

pSHKLx1 carrying a 100 bp genomic DNA fragment from M. fortuitum 10860/03 containing the beginning of the porM1 gene with the SD-sequence in antisense-orientation with respect to the hsp60 promoter

This study


Sharbati et al. BMC Microbiology 2009 9:31   doi:10.1186/1471-2180-9-31

Open Data