Table 1

Oligonucleotides used in this study

Primer designation

oligonucleotides

Target/application

Predicted product

Reference/source


CVD432F

5'-CTG GCG AAA GAC TGT ATC AT-3'

AA probe (CVD 432)

629 bp

[43]


CVD432R

5'-CAA TGT ATA GAA ATC CGC TGT T-3'


aapF

5'-CTT GGG TAT CAG CCT GAA TG-3'

aap, encoding the enteroaggregative E. coli plasmid-borne anti-aggregation protein, dispersin

310 bp

[44]


aapR

5'-AAC CCA TTC GGT TAG AGC AC-3'


aggAF

5'-TTA GTC TTC TAT CTA GGG-3'

aggA, encoding the structural subunit of aggregative adherence fimbriae I

450 bp

[17]


aggAR

5'-AAA TTA ATT CCG GCA TGG-3'


aggRF

5'-CTA ATT GTA CAA TCG ATG TA-3'

aggR, encoding the enteroaggregative E. coli plasmid-borne aggregative adherence regulator

457 bp

[44]


aggRR

5'-AGA GTC CAT CTC TTT GAT AAG-3'


M13F

5'-GGT TTT CCC AGT CAC GAC-3'

Vector priming sequencing primer

Not applicable


M13R

5'-CAG GAA ACA GCT ATG ACC-3'

Vector priming sequencing primer

Not applicable


aafBdaaDF

5'-CCTGCGGGATGTTACT-3'

aafB from EAEC and daaD from DAEC

333/339

This study


aafBdaaDR

5'-GCCATCACATCAAAAA-3'


Snelling et al. BMC Microbiology 2009 9:269   doi:10.1186/1471-2180-9-269

Open Data