Vesicles co-localize with the endoplasmic reticulum marker TRAPα. AF488-S470 vesicles (2.5 μg) were incubated with A549 cells for 1 hour at 37°C. Cell surface was labeled using biotin and AF633-streptavidin (blue). Cells were washed, fixed, permeabilized with 0.015% digitonin to release cytoplasm, and probed with anti-TRAPα primary antibody and AF555-labeled secondary antibody.
Bauman and Kuehn BMC Microbiology 2009 9:26 doi:10.1186/1471-2180-9-26