Table 2

Primer information for amplification of the fliY and bla genes.

Gene

Primer sequence (5'-3')

Product size


fliY

F: GCC

    GGA TCC
(BamH I) ATG GGT GAA GGT TCC CTA TCA CAG

1065 bp

R: GCC

    AAG CTT
(Hind III) TCA CTT ACC CTC CGG CTT AAT CCG

bla

F: GCC

    AGA TCT
(Bgl II) TCT AAA TAC ATT CAA ATA TGT

954 bp

R: GCC

    AGA TCT
(Bgl II) CTT GGT CTG ACA GTT ACC AAT

fliP

F: ATG AAA ATG AGA CAT AAA

804 bp

R: TCA TTT ATA ACT CCT TAC

fliQ

F: ATG ACG GAA TTA GAC GTT ATG

264 bp

R: CTA AAA TTT TTC GAT CAT CAA


F: forward primer, R: reverse primer.

Liao et al. BMC Microbiology 2009 9:253   doi:10.1186/1471-2180-9-253

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