Table 5

Organic acid production by fluorescent Pseudomonas during North Carolina rock phosphate solubilization.

Organic acid (μg/ml)


Strain

P-liberated (μg/ml)

Final pH

Oxalic

Gluconic

2-KGA

Lactic

Succinic

Formic

Citric

Malic

Total organic acids (μg/ml)


P. trivialis

BIHB 728

191.3 ± 1.0

3.70

14.7 ± 0.6

3810.0 ± 7.6

10.2 ± 1.0

ND

ND

ND

ND

ND

3834.9

BIHB 736

172.0 ± 0.3

3.72

9.1 ± 1.3

4672.3 ± 6.4

ND

42.7 ± 1.2

ND

ND

ND

ND

4724.1

BIHB 745

168.2 ± 0.4

3.73

10.8 ± 0.5

3880.7 ± 5.2

10.1 ± 0.8

ND

ND

ND

ND

ND

3901.6

BIHB 747

173.0 ± 0.4

3.81

16.6 ± 1.0

6035.0 ± 4.2

11.0 ± 1.8

40.3 ± 2.9

ND

ND

ND

ND

6102.9

BIHB 749

177.3 ± 0.6

3.73

17.1 ± 0.9

4587.0 ± 4.7

ND

42.7 ± 2.2

ND

ND

ND

113.2 ± 2.7

4760.0

BIHB 750

145.7 ± 1.2

3.88

10.3 ± 0.6

4395.3 ± 7.7

ND

ND

ND

ND

ND

ND

4405.6

BIHB 757

175.0 ± 0.3

3.92

13.6 ± 2.3

4649.0 ± 5.5

13.3 ± 1.1

ND

ND

ND

ND

ND

4675.9

BIHB 759

178.0 ± 0.6

3.81

11.0 ± 1.4

5331.0 ± 6.1

ND

ND

ND

ND

ND

ND

5342.0

BIHB 763

161.2 ± 0.2

3.80

11.5 ± 1.3

4362.0 ± 4.6

10.8 ± 1.0

ND

ND

ND

ND

ND

4384.3

BIHB 769

224.0 ± 0.7

3.55

10.8 ± 0.8

4448.0 ± 5.3

ND

ND

ND

ND

ND

ND

4458.8

P. poae

BIHB 730

163.8 ± 1.1

3.90

10.1 ± 1.2

3770.0 ± 6.4

ND

ND

ND

ND

ND

ND

3780.1

BIHB 752

204.3 ± 0.7

3.72

12.7 ± 1.5

4947.0 ± 6.0

10.3 ± 1.0

ND

ND

ND

26.1 ± 2.0

ND

4996.1

BIHB 808

193.4 ± 0.7

3.65

11.5 ± 1.2

4420.3 ± 2.9

10.9 ± 0.8

ND

45.1 ± 4.3

ND

ND

ND

4442.7

P. fluorescens

BIHB 740

236.8 ± 0.6

3.48

9.8 ± 1.1

4762.7 ± 4.3

31.3 ± 2.0

ND

46.7 ± 3.2

59.3 ± 3.5

ND

104.8 ± 3.0

5014.6

Pseudomonas spp.

BIHB 751

123.3 ± 1.4

3.89

9.1 ± 1.1

3241.0 ± 2.6

22.3 ± 1.9

ND

ND

ND

ND

415.0 ± 4.0

3687.4

BIHB 756

164.2 ± 0.8

3.82

11.3 ± 0.6

4975.0 ± 7.5

ND

41.7 ± 1.4

ND

ND

29.5 ± 2.2

ND

5057.5

BIHB 804

161.5 ± 1.0

3.78

15.7 ± 1.2

4542.0 ± 5.3

10.5 ± 1.0

39.3 ± 2.0

ND

ND

ND

33.0 ± 1.2

4640.5

BIHB 811

173.0 ± 1.1

3.92

15.5 ± 0.8

2549.0 ± 5.9

32.7 ± 0.9

54.3 ± 2.0

75.1 ± 4.6

ND

ND

265.0 ± 3.6

2991.6

BIHB 813

92.7 ± 1.2

4.07

8.9 ± 1.2

4633.3 ± 5.5

ND

38.8 ± 2.0

ND

ND

ND

ND

4681.0


Total organic acids (μg/ml)

230.1

84010.6

173.4

299.8

121.8

59.3

55.6

931

85881.6


Values are the mean of three replicates ± standard error of the mean; ND = Not detected; 2-KGA = 2-ketogluconic acid.

Vyas and Gulati BMC Microbiology 2009 9:174   doi:10.1186/1471-2180-9-174

Open Data