Table 2

Organic acid production by fluorescent Pseudomonas during tricalcium phosphate solubilization.

Organic acid (μg/ml)


Strain

P-liberated (μg/ml)

Final pH

Oxalic

Gluconic

2-KGA

Lactic

Succinic

Formic

Citric

Malic

Total organic acids (μg/ml)


P. trivialis

BIHB 728

771.3 ± 1.2

3.63

ND

18350.0 ± 5.8

257.0 ± 4.9

49.3 ± 1.8

987.7 ± 3.0

ND

30.5 ± 2.8

2051.8 ± 5.2

21726.3

BIHB 736

778.7 ± 2.4

3.90

ND

18035.3 ± 9.0

177.0 ± 2.6

ND

583.7 ± 4.1

96.0 ± 2.3

ND

1042.0 ± 3.8

19934.0

BIHB 745

827.4 ± 1.8

3.65

ND

18054.3 ± 8.1

210.0 ± 2.9

ND

2249.0 ± 4.4

ND

65.2 ± 2.6

1654.5 ± 3.8

22233.0

BIHB 747

743.0 ± 1.7

3.52

ND

18216.7 ± 3.5

330.7 ± 2.9

ND

1307.7 ± 4.6

ND

25.5 ± 2.1

667.0 ± 3.2

20547.6

BIHB 749

801.0 ± 2.1

3.42

ND

17745.3 ± 7.2

193.7 ± 3.3

ND

797.6 ± 1.9

117.5 ± 2.0

ND

1236.0 ± 6.2

20090.1

BIHB 750

774.3 ± 1.9

3.82

ND

18624.0 ± 4.6

172.3 ± 3.7

ND

509.9 ± 2.7

93.5 ± 1.7

ND

2012.3 ± 3.9

21412.0

BIHB 757

775.3 ± 2.3

3.92

ND

17819.0 ± 6.7

224.5 ± 2.6

ND

772.3 ± 3.4

132.0 ± 3.5

ND

911.0 ± 6.1

19858.8

BIHB 759

751.3 ± 3.7

3.72

ND

18336.3 ± 4.5

179.0 ± 2.9

ND

779.0 ± 5.0

116.0 ± 3.2

ND

2551.0 ± 4.9

21961.3

BIHB 763

718.0 ± 1.5

4.00

ND

17901.3 ± 5.9

173.7 ± 2.6

ND

659.7 ± 4.1

106.0 ± 5.0

ND

2656.0 ± 2.7

21496.7

BIHB 769

806.4 ± 2.3

3.70

ND

19340.0 ± 5.8

154.0 ± 2.5

ND

207.7 ± 3.8

ND

ND

1965.0 ± 5.1

21666.7

P. poae

BIHB 730

768.3 ± 1.8

3.40

ND

17464.7 ± 5.5

251.0 ± 3.1

ND

1172.7 ± 5.9

ND

ND

1718.8 ± 3.4

20607.2

BIHB 752

805.0 ± 1.7

3.50

ND

18800.7 ± 6.4

217.0 ± 4.2

ND

321.3 ± 4.1

ND

ND

3128.0 ± 4.5

22467.0

BIHB 808

821.4 ± 1.7

3.58

ND

18840.3 ± 7.3

176.3 ± 2.3

ND

475.7 ± 6.6

ND

44.3 ± 2.9

75.0 ± 3.6

19611.6

P. fluorescens

BIHB 740

768.3 ± 2.6

3.97

ND

17038.7 ± 3.8

175.3 ± 4.4

ND

163.3 ± 3.5

129.0 ± 3.8

46.0 ± 3.2

3178.0 ± 3.8

20730.3

Pseudomonas spp.

BIHB 751

318.7 ± 2.0

4.20

7.7 ± 0.6

216.7 ± 3.5

532.3 ± 4.3

ND

ND

23.8 ± 1.7

ND

1181.0 ± 5.9

1961.5

BIHB 756

802.3 ± 2.1

3.53

ND

17937.3 ± 6.2

378.0 ± 3.6

ND

209.4 ± 3.2

ND

ND

4215.0 ± 3.2

22739.7

BIHB 804

805.1 ± 2.2

3.55

ND

17929.7 ± 4.1

122.7 ± 2.4

53.7 ± 1.8

96.0 ± 2.5

ND

ND

1520.0 ± 3.8

19722.1

BIHB 811

717.3 ± 1.9

3.98

ND

14427.3 ± 2.3

14.3 ± 0.4

ND

195.3 ± 4.3

ND

28.5 ± 1.8

ND

14665.4

BIHB 813

631.7 ± 2.5

3.93

ND

18057.7 ± 5.4

175.3 ± 5.9

ND

536.3 ± 4.5

114.4 ± 4.4

ND

913.7 ± 3.7

19797.4


Total organic acids (μg/ml)

7.7

323135.3

4114.1

103.0

12024.3

928.2

240.0

32676.1

373228.7


Values are the mean of three replicates ± standard error of the mean; ND = not detected; 2-KGA = 2-ketogluconic acid.

Vyas and Gulati BMC Microbiology 2009 9:174   doi:10.1186/1471-2180-9-174

Open Data