Table 3

Additional amino acid substitutions identified by ERG11 sequencing in five C. albicans isolates with reduced susceptibility to fluconazole.

Patient/isolate no.

Substitutions detected by RCA

Substitutions detected by DNA sequencing

5

G307S

G307S, G450V

6-A^a

E266D

E266D, D153E

6-B^a

D116E

D116E, D153E

10

E266D, V488I, S405F, Y132H

E266D, V488I, S405F, Y132H, K108E

11

E266D, V437I

E266D, V437I, F126L

12-A^a

G464S

G464S, K108E

^a The "A" and "B" notation of patient numbers refers to isolates which were cultured sequentially from the same patient at different times.

Wang et al. BMC Microbiology 2009 9:167   doi:10.1186/1471-2180-9-167