Table 5

Kinetic parameters of Arthrobacter sp. 32c β-D-galactosidase.

Substrate

Temperature [°C]

K_m [mM]

k_cat [s^-1]

k_cat/K_m [s^-1mM^-1]

ONPG

10

5.75 ± 0.34

52.4 ± 0.72

9.12 ± 0.71

20

4.86 ± 0.37

81.0 ± 1.03

16.67 ± 1,60

30

3.46 ± 0.29

123.9 ± 1.21

35.81 ± 3.66

40

3.15 ± 0.27

169.9 ± 1.44

53.92 ± 5.56

50

2.62 ± 0.21

212.4 ± 1.67

81.07 ± 7.76

55

5.11 ± 0.32

71.2 ± 0.98

13.93 ± 1.14

lactose

10

77.54 ± 1.77

1.76 ± 0.11

0.023 ± 0.002

20

67.82 ± 1.74

2.36 ± 0.14

0.035 ± 0.003

30

52.67 ± 1.71

4.81 ± 0.22

0.091 ± 0.007

40

44.31 ± 1.73

5.73 ± 0.21

0.129 ± 0.010

50

39.73 ± 1.72

6.98 ± 0.23

0.176 ± 0.014

Hildebrandt et al. BMC Microbiology 2009 9:151   doi:10.1186/1471-2180-9-151