Research article
Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis
-
* Corresponding author: John A Leigh leighj@u.washington.edu
1 Department of Chemical Engineering, Box 355014, University of Washington, Seattle, WA 98195, USA
2 Department of Microbiology, Box 357242, University of Washington, Seattle, WA 98195, USA
3 University of Wisconsin-Madison, Department of Chemistry, Madison, WI 53706, USA
BMC Microbiology 2009, 9:149 doi:10.1186/1471-2180-9-149
Published: 23 July 2009Additional files
Additional file 1:
Complete list of protein abundance ratios, p-values, and q-values. Complete data set, with log2 ratios, p-values, q-values, and abundance trends (up, down, or no significant difference).
Format: XLS Size: 1.4MB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 2:
Proteins with altered abundance under H2 limitation. Log2 ratios for proteins with altered abundance under H2 limitation.
Format: XLS Size: 76KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 3:
Proteins with altered abundance under nitrogen limitation. Log2 ratios for proteins with altered abundance under nitrogen limitation.
Format: XLS Size: 72KB Download file
This file can be viewed with: Microsoft Excel Viewer
Additional file 4:
Proteins with altered abundance under phosphate limitation. Log2 ratios for proteins with altered abundance under phosphate limitation. Summary tables of individual proteomic results at the peptide level, in the form of DTASelect Ver. 1.9 filter files [23], are posted at http://depts.washington.edu/mhlab/Mm900nutrientlimitation webcite. Log in with user name MMP and password threebugs. These files are organized by the archive names given under Materials and Methods and contain Sequest [22] scores for individual peptide mass spectra, search parameters and other detailed information that can be used to assess data quality at multiple levels, i.e. peptides, proteins and individual CID (MS2) mass spectra. The sequest.params file for each analysis is also posted. Researchers interested in the raw data (*.RAW files) should contact mhackett@u.washington.edu.
Format: XLS Size: 83KB Download file
This file can be viewed with: Microsoft Excel Viewer