Table 3

Comparison of phage titer determinations with DLA and with PAMA using different antibiotics

DLA

AMP [0.5]

CEF [0.06]

TET [1.5]


phi PVP-SE1


PFUs (average ± SD)

14 ± 5

55 ± 10

53 ± 11

58 ± 10


SD %

38

19

21

17


phi PVP-SE2


PFUs (average ± SD)

54 ± 4

54 ± 5

48 ± 11

51 ± 3


SD %

8

8

23

6


DLA: classical Double-Layer Agar technique; AMP [0.5]: PAMA with 0.5 mg/l ampicillin; CEF [0.06]: PAMA with 0.06 mg/l cefotaxime; TET [1.5]: PAMA with 1.5 mg/l tetracycline.

Santos et al. BMC Microbiology 2009 9:148   doi:10.1186/1471-2180-9-148

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