Figure 1.

Phylogenetic relationships of sequence types of 95 strains of attaching-effacing E. coli. An unrooted phylogenetic tree was constructed by the neighbour-joining algorithm based on the Kimura two-parameter model of nucleotide substitution. Bootstrap values greater than 50% based on 500 replications are given at the internal nodes. Strain names highlighted in pink are reference strains of typical EPEC or STEC; those highlighted in pale blue and yellow were originally isolated from cattle and rabbits, respectively, those highlighted in green were from children with persistent diarrhoea, and those highlighted in grey were from humans without diarrhoea. The right hand column indicates distinctive aEPEC clades in boldface type. The intimin and flagella type is shown for each clade. Abbreviations: nt, non-typable; int, intimin; ND, not determined.