Validation of the up-regulation of five heat shock protein genes (PINA1058, PINA1756, PINA1797, PINA1798, PINA2006) in strain 17 by quantitative real-time RT-PCR. Total RNA was isolated from 12 h-old cultures of strains 17 and 17-2, and the expression levels of these genes were compared by real-time RT-PCR. The average of increased expression level of PIN 0281 was less than twofold in the real-time RT-PCR analysis though a three-fold up-regulation of this gene was observed by the microarray assay.
Yamanaka et al. BMC Microbiology 2009 9:11 doi:10.1186/1471-2180-9-11