Enhancement of sensitivity of pathogen DNA detection by microarray by LSplex amplification. Hybridization profile of non-amplified genomic S. aureus DNA (2 μg, 100 ng, 10 ng and 1 ng) and indirectly labelled LSplex amplification product of the same DNA starting from 10 ng, 1 ng and 0.1 ng template (columns). Each row represents individual S. aureus-specific capture probes as well as positive (16S-derived probes) and negative controls. Fluorescent signals were quantified and classified as positive (black boxes) hybridization or absence of hybridization (white boxes).
Palka-Santini et al. BMC Microbiology 2009 9:1 doi:10.1186/1471-2180-9-1