Microarray detection of LSplex amplification products labelled by different techniques: Hybridization pattern of specific capture probes obtained upon hybridization of 2 μg (A) and 10 ng of S. aureus DNA (B) served as standard for comparison of the profiling fidelity and sensitivity of three labelling protocols for LSplex. LSplex amplification of 10 ng S. aureus DNA with subsequent labelling by random priming (C). Direct incorporation of Chromatide Alexa Fluor 546-47-dUTPs during LSplex amplification (D). Indirect labelling by incorporating amino-modified nucleotides during LSplex and subsequent coupling with amino reactive dyes (E).
Palka-Santini et al. BMC Microbiology 2009 9:1 doi:10.1186/1471-2180-9-1