Open Access Highly Accessed Methodology article

Large scale multiplex PCR improves pathogen detection by DNA microarrays

Maria Palka-Santini1, Berit E Cleven12, Ludwig Eichinger3, Martin Krönke14 and Oleg Krut14*

Author Affiliations

1 Institute for Medical Microbiology, Immunology and Hygiene, Medical Faculty, University of Cologne, Germany

2 Vulkan Technic Maschinen-Konstruktions GmbH, Germany

3 Center for Biochemistry, Medical Faculty, University of Cologne, Germany

4 Center for Molecular Medicine Cologne, Medical Center, University of Cologne, Germany

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BMC Microbiology 2009, 9:1  doi:10.1186/1471-2180-9-1

Published: 3 January 2009

Additional files

Additional file 1:

Microarray probes and primer sequences. The table contains the description of microarray probes and primer sequences used in the study.

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Additional file 2:

Prototype DNA microarray for detection of common pathogens. The figure represents the analysis of microarray hybridizations with decreasing amounts of bacterial DNA.

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