Figure 1.

Sequences of selected Lrp proteins. Lrp proteins from various bacterial species were aligned; species used in this study are in bold (Escherichia coli, Proteus mirabilis, and Vibrio cholerae). A more complete list of Lrp orthologs and paralogs can be found online [140]. The gray-shaded regions indicate N- and C-terminal sequences that are conserved among enterobacterial Lrp orthologs, and the black-shaded regions indicate substitutions relative to E. coli. The boxed regions indicate the DNA-binding helix-turn-helix motif (top portion, under cartoon representation), and the leucine-binding sites (lower portion of sequence); all boxed regions are completely conserved among the species used in this study. For references, see main text. Other Lrp orthologs shown came from (in order shown): Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae, Yersinia pestis, Haemophilus influenzae Rd, Actinobacillus pleuropneumoniae, Pasteurella multocida, and Pseudomonas aeruginosa.