Table 2

Major phylotypes obtained from the cellulose-fed MFCa

Major phylotype

No. of clone in each libraryb

Database match (Accesion no.)


Soil (day 0)

Phase I (day 46)

Phase III (day 137)

Phase IV (day 216)

Phase V (day 306)


Firmicutes

Mfc-1

0

32

25

5

4

95% Ethanoligenens harbinense YUAN-3 (AY295777)

Mfc-2

0

3

5

9

7

95% Clostridium sp. Z6 (AY949859)

Mfc-3

0

0

5

9

11

94% Propionispora hapie (AJ508928)

Mfc-4

0

43

0

0

0

88% Clostridium sp. JC3 (AB093546)

Mfc-5

0

0

0

2

22

99% Clostridium celerecrescens (X71848)

Othersc

5

7

4

6

7

Chloroflexi

Mfc-6

0

3

6

19

22

92% Leptolinea tardivitalisYMTK-2 (AB109438)

Othersc

5

0

0

0

0

Bacteroidetes

Mfc-7

0

22

5

5

3

91% Paludibacter propionicigens (AB078842)

Othersc

6

7

1

0

0

Spirochaetes

Mfc-8

0

6

4

21

6

92% Treponema denticola ATCC35405 (AE017226)

Othersc

0

4

0

0

0

Proteobacteria

Mfc-9

0

0

1

23

22

97% Rhizobiales bacterium RR54 (AB174822)

Mfc-10

0

3

7

1

3

96% Myxobacterium sp. KC (AF482687)

Soil-1

9

0

0

0

0

99% Achromobacter xylosoxidans (AF411021)

Soil-2

7

0

0

0

0

99% Stenotrophomonas maltophilia E2 (AY841799)

Othersc

26

0

1

5

2

Euryarchaeota

Mfc-11

0

2

8

10

9

99% Methanobacterium bryantii RiH2 (AF028688)

Othersc

0

1

2

0

2

Other phyla and unknown clones

Othersc

34

9

1

0

5


Total clone

93

142

75

115

129


Ratio (%)d

17

80

88

90

85


a The Major phylotype was defined as a phylotype containing more than 5 clones.

b Names for the libraries correspond to the subculture phases of MFC.

c For a list of other phylotypes (minor phylotypes), refer to the supplementary materials that may be found in the website (Table S1).

d Ratio in number of clones affiliated with the major phylotypes to the total clones.

Ishii et al. BMC Microbiology 2008 8:6   doi:10.1186/1471-2180-8-6

Open Data