Table 2

Oligonucleotide primers used in this study

Primer

Sequence

Reference or source


gap3-gfp-F

5'-CGGCCGTCGACATGACTAAACAGTTAATTTC-3'

This study

gap3-gfp-R

5'-CGCCGCGGTACCAATATATTCTATTAAATTTTTC-3'

This study

Δ62–67 Upstream

5'-GCAGACTCGAGAGAATGACGATCACCTTG-3'

This study

Δ62–67 Downstream

5'-CGGGCCTCGAGATACAAGGAAATAGATACCTTC-3'

This study

Δ144–157 Upstream

5'-GCCCGCCCTCGAGATCATAAATCATAAATTTTTG-3'

This study

Δ144–157 Downstream

5'-TATCTCTCGAGTATCTACGGGAACTGCTTCCG-3'

This study

Val33Arg-F

5'-CCAAGAGGATTTAAGGAATTTTTACAATCCACAGCTCGC-3'

This study

Val33Arg-R

5'-GCGAGCTGTGGATTGTAAAAATTCCTTAAATCCTCTTGG-3'

This study

Phe35His-F

5'-CCAAGAGGATTTAGTGAATCATTACAATCCACAGCTCGC-3'

This study

Phe35His-R

5'-GCGAGCTGTGGATTGTAATGATTCACTAAATCCTCTTGG-3'

This study

Asn37Ile-F

5'-GGATTTAGTGAATTTTTACATTCCACAGCTCGCATCAGGG-3'

This study

Asn37Ile-R

5'-CCCTGATGCGAGCTGTGGAATGTAAAAATTCACTAAATCC-3'

This study

Pro38Arg-F

5'-GTGAATTTTTACAATCGACAGCTCGCATCAGG-3'

This study

Pro38Arg-R

5'-CCTGATGCGAGCTGTCGATTGTAAAAATTCAC-3'

This study

Ser42Leu-F

5'-CAATCCACAGCTCGCATTAGGGAAGCCTATTG-3'

This study

Ser42Leu-R

5'-CAATAGGCTTCCCTAATGCGAGCTGTGGATTG-3'

This study

Asn54 Ile-F

5'-GTCACGAACAATTTATCAAGGTGATCGTCATTCTCC-3'

This study

Asn54 Ile-R

5'-GGAGAATGACGATCACCTTGATAAATTGTTCGTGAC-3'

This study

Pro62Gly-F

5'-GGTGATCGTCATTCTGGAGATCTTCCAATTC-3'

This study

Pro62Gly-R

5'-GAATTGGAAGATCTCCAGAATGACGATCACC-3'

This study

Asp63Val-F

5'-GGTGAT CGTCATTCTCCAGTTCTTCCAATTCTTATACAAGG-3'

This study

Asp63Val-R

5'-CCTTGTATAAGAATTGGAAGAACTGGAGAATGACG ATCACC-3'

This study

Leu64Arg-F

5'-CGTCATTCTCCAGATCGTCCAATTCTTATACAAGG-3'

This study

Leu64Arg-R

5'-CCTTGTATAAGAATTGGACGATCTGGAGAATGACG-3'

This study

Pro65Arg-F

5'-CGTCATTCTCCAGATCTTCGAATTCTTATACAAGG-3'

This study

Pro65Arg-R

5'-CCTTGTATAAGAATTCGAAGATCTGGAGAATGACG-3'

This study

Ile66Asn-F

5'-CGTCATTCTCCAGATCTTCCAAATCTTATACAAGG AAATAG-3'

This study

Ile66Asn-R

5'-CTATTT CCTTGTATAAGATTTGGAAGATCTGGAGAATGACG-3'

This study

Leu67Thr-F

5'-CGTCATTCTCCAGATCTTCCAATTACGATACAAGGAAATAG-3'

This study

Leu67Thr-R

5'-CTATTTCCTTGTATCGTAATTGGAAGATCTGGAGAATGACG-3'

This study

Leu75Ser-F

5'-GGAAATAGATACCTTTCAGAGTATCAGATTGATGCTGTACC-3'

This study

Leu75Ser-R

5'-GGTACAGCATCAATCTGATACTCTGAAAGGTATCTATTTCC-3'

This study

Arg59Leu-F

5'-CGAACAAATTATCAAGGTGATCTTCATTCTCCAGATCTTCC-3'

This study

Arg59Leu-R

5'-GGAAGATCTGGAGAATGAAGATCACCTTGATAATTTGTTCG-3'

This study


Peng et al. BMC Microbiology 2008 8:52   doi:10.1186/1471-2180-8-52

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