Cell surface expression of Fap1 determined by BactELISA. MAbs F51 and E42 were used in BactELISA analysis to determine Fap1 expression in wild type FW213, fap1 mutant VT1393, gap3 mutant VT1619, gap3 complemented strain gap3-/gap3+ and Gap3 site-directed mutants, L64R, P65R, I66N and L67T. Data were obtained from three experiments in triplicates and are presented as means ± standard deviation.
Peng et al. BMC Microbiology 2008 8:52 doi:10.1186/1471-2180-8-52