Table 5

Phenotypic characteristics of isolates demonstrating previously unreported PRA-hsp65 patterns.

Species

PRA hsp65

25°C

37°C

45°C

pg

TCH

nit

Tween

NaCl

Aryl3

Aryl15

ag

pic

β-gal

LJ

HA

PNB

INH

RF

EMB

CIP

OFL


M. arupense

NP17

3

3

0

N

2–3

0

1

0

0–1

0–2

nd

0

0–1

nd

3

2–3

3

0

0

0–1

3

M. avium

NP10

3

3

0

N

3

3

1

1

2

3

3

0

0

3

3

1

1

1

0

0

0

M. cosmeticum

NP6

3

3

2

N

3

0

1

0

1

2

1

2

1

nd

2

3

2

3

2

1

2

M. fortuitum

NP12

3

3

0

S

3

3

1

3

1

3

2

1

0

3

3

3

3

3

3

0

3

M. fortuitum

NP19

3

3

0

N

3

3

0

3

3

3

3

3

1

3

3

3

3

3

3

0

2

M. gordonae

NP1

2–3

3

0

S

3

0

1

0

1

2

0

0

0

3

1–3

3

0–3

0–3

0–1

0–1

1–3

M. gordonae

NP3

3

1

0

S

3

0

2

0

0

2

0

0

0

3

1

2

0

0

0

0

0

M. gordonae

NP13

1

2

0

P

3

1

0

nd

0

1

0

0

0

3

3

3

0

1

1

2

1

M. gordonae

NP22

3

3

3

S

3

3

2

0

0

0

nd

0

0

3

0

3

1

3

3

0

nd

M. mageritense

NP5

3

3

0

N

3

2

1

0

0

2

3

0

0

3

3

3

0

3

0

0

0

M. nonchromogenicum

NP4

2

3

2

N

2

1

1

0

0

0

nd

1

1

nd

2

2

2

2

0

0

0

M. sherrisii

NP14

1

2

0

S

1–2

0

0

1–2

0

0

1

0

1

2

1–2

1–2

1

1

2

2

1–2

M. terrae

NP11

2

3

0

N/S

2–3

0–3

1, 2

1

0

0–1

0

0

0–1

3

3

1, 2

3

0

0

0, 1

3


Phenotypes: 24°C, 36°C, 45°C: growth at temperature shown; pg: pigmentation (N, nonchromogen; P, photochromogen; S, scotochromogen); TCH: growth on thiophene-2-carboxylic acid hydrazide; nit: nitrate reduction; Tween: hydrolysis of Tween 80; NaCl: growth on 5% NaCl; Aryl 3. Aryl 15: arylsulfatase activity after 3 and 15 days of growth, respectively; ag: growth on nutrient agar; pic: growth on picric acid; β-gal: β-galactosidase activity; LJ: growth on Löwenstein-Jensen media; HA: growth on hydroxylamine 500 μg/ml; PNB: growth on p-nitrobenzoic acid; INH: isoniazid; RF: rifampicin; EMB: ethambutol; CIP: ciprofloxacin; OFL: ofloxacin. Responses are graded 0 (negative, no growth, no activity expressed) to 3 (positive, heavy growth, strong activity expressed); nd, not done. For patterns with multiple isolates, the result shown represents the most common phenotype(s) or the range of phenotypes observed.

Chimara et al. BMC Microbiology 2008 8:48   doi:10.1186/1471-2180-8-48

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