Table 4

BstEII and HaeIII fragment lengths (base pairs) for 30 isolates with new patterns by PRA-hsp65.

Speciesa

PRA-hsp65

N

Fragment BstEII

Length (bp) HaeIII


M. arupense

NP17

5

320-115

145-75-60

M. avium

NP10

1

320-115

140-90-60

M. cosmeticum

NP6

1

320-115

150-95-80

M. fortuitum

NP12

1

235-120-85

140-120-100-55

M. fortuitum

NP19

1

235-120-100

145-140-100-55

M. gordonae

NP1

11

235-120-100

130-110-95

M. gordonae

NP3

1

320-130

130-60

M. gordonae

NP13

1

235-120-85

130-90

M. gordonae

NP22

1

235-130-85

160-90-60

M. mageritense

NP5

1

240-130-85

145-100-50

M. nonchromogenicum

NP4

1

235-120-85

145-80-60

M. sherrisii

NP14

3

235-120-85

145-130

M. terrae

NP11

2

235-210

140-115-70


a Species identification based on sequencing of hsp65 gene. Bold indicates sequences submitted to GenBank and patterns included in the updated PRA-hsp65 algorithm (see Figures 1, 2 and 3). GenBank accession numbers: NP1, EF601222; NP11, EF601223; NP14, AY365190 [23]; NP17, DQ168662 [18].

All isolates with new PRA-hsp65 profiles were cultured from sputum, with the following exceptions: NP1: urine (2), feces, liver biopsy and unknown (one each); NP17: unknown (2).

Chimara et al. BMC Microbiology 2008 8:48   doi:10.1186/1471-2180-8-48

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