Table 1

Primers used in this study.

Primer

Purpose

Sequence (5' to 3') a,b

Product size (bp)


repA-UNI-5

universal repA PCR

GGA TGT GGT GAG TGC CAG TTC A

898

repA-UNI-3

universal repA PCR

CCG CTG YTC GGT CAT CTG C

repA-SEQ-5

repA sequencing species-specific PCR

CAG CAG GCC GAC GAC TCG

623

repA-SEQ-3

repA sequencing

ATC GGC TGC TTG CGY TCG GT

repA-636-3

species-specific PCR

GTC GAG CGC

    G
AG CAT CGG
    C

636

repA-237-3

species-specific PCR

CCA

    C
AC GCG
    G
CG
    G
GT
    GGT

237

parB-UNI-5

universal parB PCR

parB sequencing

GCA GCA GGC GAT YCA CGT G

656

parB-UNI-3

universal parB PCR

parB sequencing

CGT CGC GCT TST CCT TCG G


a Standard code, Y = C or T; S = C or G

b Mis-matched nucleotide positions in the species specific primers are underlined

Drevinek et al. BMC Microbiology 2008 8:44   doi:10.1186/1471-2180-8-44

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