PCR products identifying B. cenocepacia produced by the multiplex repA PCR. Double band positivity was characteristic for B. cenocepacia IIIA, IIIB and IIID groups. All other species of Bcc and novel MLST-defined groups were either positive for one band or double negative (Table 2). Samples analysed in each lane are as follows (isolate number): M, 1 kb molecular size marker (relevant size fragments indicated in bp); 1, B. cepacia (BCC0002); 2, B. multivorans (BCC0814); 3, B. cenocepacia IIIA (BCC1295); 4, B. cenocepacia IIIB (BCC0187); 5, B. cenocepacia IIIC (BCC0631); 6, B. cenocepacia IIID (BCC0506); 7, Bcc Group E (BCC0198); 8, B. stabilis (BCC0479); 9, B. vietnamiensis (BCC0195); 10, B. dolosa (BCC0161); 11, B. ambifaria (BCC0363); 12, B. anthina (BCC0036); 13, B. pyrrocinia (BCC0488); 14, Bcc Group K (BCC1303); 15, BCC2 (BCC0484); 16, BCC3 (BCC1306); 17, BCC4 (BCC0405) and 18, BCC5 (BCC0397).
Drevinek et al. BMC Microbiology 2008 8:44 doi:10.1186/1471-2180-8-44