Table 7

Results obtained for diabetic foot ulcer sample (D) using shotgun Sanger sequencing (FRACS).

Genus species

Seq

%

Gram

Aerotolerance

Shape


Staphylococcus aureus *

70

39.33

+

facultative

Cocci

Anaerococcus lactolyticus

16

8.99

+

anaerobic

Cocci

Anaerococcus vaginalis

15

8.43

+

anaerobic

cocci

Bacterioides fragilis

7

3.93

-

anaerobic

rod

Finegoldia magna

6

3.37

+

anaerobic

cocci

Morganella morganii

5

2.81

-

Facultative anaerobe

rod

Enterococcus faecalis*

5

2.81

+

facultative

cocci

Peptoniphilus harei

5

2.81

+

anaerobic

cocci

Clostridium novyi

4

2.25

+

anaerobic

rod

Veillonella atypica

4

2.25

-

anaerobic

cocci

Abiotrophia para-adiacens

4

2.25

+

anaerobic

cocci

Veillonella parvula

4

2.25

-

anaerobic

cocci

Citrobacter murliniae *

3

1.69

-

facultative anaerobe

rod

Haemophilus spp.

3

1.69

-

facultative

rod

Clostridium spp.

3

1.69

+

anaerobic

rod

Haemophilus segnis

3

1.69

-

faculative

rod

Enterococcus avium*

3

1.69

+

facultative

cocci

Proteus spp.*

2

1.12

-

Facultative anaerobe

rod

Enterobacter aerogenes

2

1.12

-

facultative

rod

Dialister spp.

2

1.12

-

anaerobic

rod

Peptoniphilus asaccharolyticus

2

1.12

+

anaerobic

cocci

Enterobacter cloacae

1

0.56

-

Facultative anaerobe

rod

Klebsiella oxytoca*

1

0.56

-

Facultative anaerobe

rod

Escherichia coli

1

0.56

-

facultative

rod

Pseudoalteromonas spp.

1

0.56

-

facultative

rod

Porphyromonas levii

1

0.56

-

anaerobic

rod

Delftia acidovorans

1

0.56

-

aerobic

rod

Dialister invisus

1

0.56

-

anaerobic

rod

Staphylococcus epidermidis*

1

0.56

+

facultative

cocci

Staphylococcus spp.*

1

0.56

+

facultative

cocci

Granulicatella spp.

1

0.56

+

anaerobic

cocci


This table provides the identified genera of the bacteria found using shotgun Sanger sequencing (FRACS) in the D sample, the number of sequences corresponding to this genus, the known gram staining properties, the aerotolerance (anaerobic, anaerobic, or facultative anaerobic) nature of the genus, and the shape of the genus. The asterisk * identifies bacteria previously cultured from a single culture result from each subject's medical record from subjects in the D pool. Bacteria that were cultured and were not found using FRACSS include Pseudomonas, Serratia, and Streptococcus. The % represents the percentage of the total sequences analyzed within the sample.

Dowd et al. BMC Microbiology 2008 8:43   doi:10.1186/1471-2180-8-43

Open Data