Phylogenetic analyses of phosphate solubilizing strains of fluorescent pseudomonads based on the nucleotide sequence of 16S rRNA. The multiple sequence alignment was done in CLUSTAL program. The pair-wise evolutionary distances were calculated using Kimura2-parameter model. The phylogenetic tree was constructed by neighbor-joining (NJ) method with 1000 replicates using bootstrap. A total of 7 reference fluorescent pseudomonad strains were used for the tree construction.
Naik et al. BMC Microbiology 2008 8:230 doi:10.1186/1471-2180-8-230