Table 2 |
||
|
Primer combinations used to validate each fimbrial mutation. |
||
|
Primer combination |
Predicted amplicon size (bp) |
Sequence (5'-3') |
|
|
||
|
bcfAFOR + C1 |
633 |
TGCACTATCCGCAACGATATATTT |
|
|
||
|
bcfAREV + C2 |
507 |
TAAAATACGCTTTCGCGATCGGTCGGT |
|
|
||
|
csgAFOR + C2 |
173 |
CAAGGAGCAATAAAGTATGCATAATTT |
|
|
||
|
csgAREV + C1 |
302 |
CAGCAGTTGTAGTGCAGAAACAGTCGCATA |
|
|
||
|
lpfAFOR + C2 |
867 |
TTAGTTACGCGCTGTGTCAA |
|
|
||
|
lpfAREV + C1 |
288 |
ATCCAATACCCACCTCTATACACTCCA |
|
|
||
|
fimAFOR +C1 |
807 |
AACCTCAGATCGCACCTGCTGC |
|
|
||
|
fimAREV + C2 |
429 |
ATGCCGACATGACGCCAGACC |
|
|
||
|
sefAFOR + C1 |
373 |
CTATTAATGGGGATGTTGTGTAA |
|
|
||
|
sefAREV + C2 |
946 |
CTAATAATCTCTTATAATTTC |
|
|
||
|
safAFOR + C1 |
701 |
TGAGACTCTCTCATTGGAGCGCT |
|
|
||
|
safAREV + C2 |
597 |
AATTGAGGTCAAGGGTCGCGCC |
|
|
||
|
stbAFOR + C2 |
887 |
TTAATGGTGGGGGACATCGTA |
|
|
||
|
stbAREV + C1 |
295 |
TTATTTTTACCACTCCATAAGCACGAA |
|
|
||
|
pegAFOR + C2 |
179 |
CACAAGCCAGGCATAATGCAATCATC |
|
|
||
|
pegAREV + C1 |
377 |
ACATTGCGATAACTTCCTGTCTATGAGAA |
|
|
||
|
stdAFOR + C2 |
587 |
GCTGTACCGTACCTGACTGTC |
|
|
||
|
stdAREV + C1 |
714 |
TGTTTTTAAATTTCATCCGCGAAG |
|
|
||
|
steAFOR + C1 |
739 |
TACGACAACGCCTATATAATA |
|
|
||
|
steAREV + C2 |
600 |
AGCAGCGTGGAGTGTCCCAGGTCAGC |
|
|
||
|
stfAFOR + C1 |
283 |
CATATAAACATGGGGTATTGATGA |
|
|
||
|
stfAREV + C2 |
155 |
GGCTGGCATCATCTTTAACA |
|
|
||
|
sthAFOR + C1 |
584 |
GCGTTGATTTTGTTAATGC |
|
|
||
|
sthAREV + C2 |
704 |
GAAAGCTCACGATTTGAGATCAAC |
|
|
||
|
stiAFOR + C2 |
385 |
TTTGGCCGACAACACACTATG |
|
|
||
|
stiAREV + C1 |
661 |
GTAAATCAGCTTAAATTCCG |
|
|
||
|
C1 |
- |
TTATACGCAAGGCGACAAGG |
|
|
||
|
C2 |
- |
GATCTTCCGTCACAGGTAGG |
|
|
||
|
Primers are specific to the flanking regions of the specified fimbrial gene (FOR or REV) or the chloramphenicol resistance cassette (C1 – forward or C2 – reverse). |
||
|
Clayton et al. BMC Microbiology 2008 8:228 doi:10.1186/1471-2180-8-228 |
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