Table 1

Reproducibility of F-BOX-PCR analysis with HEX and 6-FAM dyes calculated as values of Jaccard's coefficient, in comparison with standard BOX-PCR with separation in agarose gel.

Strain

Agarose gel

HEX-F-BOX

6-FAM-F-BOX




DNA extraction

PCR

Gel

Overall

DNA extraction

PCR

Injection

Overall

DNA extraction

PCR

Injection

Overall


B. cereus BC360

0.822

0.554

0.651

0.300

0.985

0.985

1.000

0.971

1.000

1.000

1.000

1.000

CI1-23

n.d.a

0.906

0.906

0.812

n.d.

n.d.

n.d.

n.d.

n.d.

0.972

0.972

0.972

CI2-13

0.864

0.952

0.952

0.818

n.d.

0.838

0.983

0.823

0.970

0.994

0.994

0.951

CI2-17

n.d.

0.624

0.811

0.500

n.d.

0.944

0.981

0.926

n.d.

0.961

0.980

0.942

CI2-23

0.907

0.905

0.864

0.727

0.961

0.983

0.994

0.933

0.969

0.969

1.000

0.939

CO2-33

n.d.

0.830

0.826

0.750

n.d.

n.d.

n.d.

n.d.

n.d.

0.978

1.000

0.978

DS3

0.791

0.597

0.803

0.375

0.971

0.971

0.990

0.923

0.919

0.979

0.992

0.950

E. coli DSM50902

0.927

0.874

0.879

0.737

0.979

0.964

0.993

0.943

0.919

0.977

0.992

0.882


Average

0.862

0.780

0.837

0.627

0.974

0.948

0.990

0.920

0.955

0.979

0.991

0.952

Std. Deviation

0.057

0.161

0.090

0.205

0.010

0.056

0.007

0.051

0.035

0.013

0.010

0.035


a n.d. not determined

Brusetti et al. BMC Microbiology 2008 8:220   doi:10.1186/1471-2180-8-220

Open Data