Determination of the optimum washing temperature for the short oligonucleotide probes. Discrimination of the perfect match probe (BLON135a), against either the 1 nt (BLON135b) (grey bars) or 2 nt (BLON135c) (white bars) mismatch probes. Labelled 16S ribosomal DNA from a pure culture of Bifidobacterium longum was hybridised at 58°C and washed between 55°C and 66°C. Perfect match probe intensity at each different temperature was then divided either by the 1 nt or 2 nt corresponding mismatch binding, and error bars represent the standard error.
Harrington et al. BMC Microbiology 2008 8:195 doi:10.1186/1471-2180-8-195