Additional file 1.

Video of series of Z-sections collected during immunocytochemistry of C. trachomatis L2 infected HEp-2 cell stained with HtrA polyclonal sera at 20 h PI (Alexa Fluor 488). The video demonstrates the staining of HtrA was around the edge of each RB within this inclusion. The staining around the periphery of each RB was consistently observed at this time point indicating a likely periplasmic location of HtrA. Scale bar 10 μM.

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Huston et al. BMC Microbiology 2008 8:190   doi:10.1186/1471-2180-8-190