Confocal microscopy images of dual labelled immunofluorescence of HtrA and LPS during C. trachomatis L2 HEp-2 cultures. The figure shows immunocytochemistry of acute, penicillin persistent, and IFN-γ persistent cultures fixed and stained with HtrA and LPS antibodies at 20 h and 44 h post infection. The LPS antibody (shown in green, column 1) has a FITC label directly conjugated to the antibody, the HtrA antibody (shown in red, second column) was detected using a secondary antiRabbit IgG antibody with an Alexa Fluor label (561). Overlays are shown in the third column. Scale bars (10 μM) are shown in the bottom right of each figure.
Huston et al. BMC Microbiology 2008 8:190 doi:10.1186/1471-2180-8-190