Figure 4.

Immunofluorescence intensity of HtrA is much higher in heat shocked C. trachomatis. Immunocytochemistry on C. trachomatis L2 infected HEp-2 cells using antibodies to HtrA and MOMP. Immunofluorescence images collected using confocal microscopy with insert showing intensity differences (glow scale) from each experiment to demonstrate differences. Samples are (A) C. trachomatis L2 infected HEp-2 cells acute (23 h PI) (HtrA polyclonal sera). (B) C. trachomatis L2 infected HEp-2 cells heat shocked for 3 h at 42°C at 20 h PI (HtrA polyclonal sera) (23 h PI). (C) C. trachomatis L2 infected HEp-2 cells acute (23 h PI) (MOMP mAb). (D)C. trachomatis L2 infected HEp-2 cells heat shocked for 3 h at 42°C at 20 h PI (MOMP mAb). The inserts in the top right show relative intensities and were recorded under identical microscope and laser settings, on the same day, to enable comparison of levels of fluorescence. Scale bars (5 μM) are shown in the bottom left corner of each figure.

Huston et al. BMC Microbiology 2008 8:190   doi:10.1186/1471-2180-8-190
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