Validation of specific primers generated by PrimerSNP. A total of 107 specific primers (amplicon ranges from 200 to 300 bp) of 9a5c, Temecula-1 and Dixon strains were tested using standard PCR. Each pair of the specific primer is displayed according to its weight score (x-axis) and the free energy of melting (ΔG°) (y-axis). The red dots represent those primers that are not specific, and blue dots represent specific primers.
Yao et al. BMC Microbiology 2008 8:185 doi:10.1186/1471-2180-8-185