Table 3

Allelotyping PCR scheme for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

O-multiplex

H1-multiplexes1

H2-multiplex

Serovars

Sensitivity5

Specificity5


B

i

I2

Typhimurium

1.00

1.00

B

r

I

Heidelberg

1.00

1.00

C2

z10

II3

Hadar

1.00

1.00

A/D1

g,m

-4

Enteritidis

1.00

0.96


1Identifies H1 alleles i; g,m; r; or z10

2 Covers H2 alleles 1,2; 1,5; 1,6; and 1,7

3 Covers H2 alleles e,n,x and e,n,z15

4 PCR negative for H2-multiplex

5Sensitivity and specificity of the allelotyping PCR scheme relative to conventional serotyping in identifying S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium among the 239 isolates examined in this study.

Hong et al. BMC Microbiology 2008 8:178   doi:10.1186/1471-2180-8-178

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