Table 2

Comparison of multiplex PCR to serotyping for identifying Salmonella O alleles B; C1; C2; D1 or E1; H1 alleles i; g,m; r or z10;and H2 alleles 1,2 or e,n,x

Phase 1 PCRs

Phase 2 multiplex PCR


Antigenic Formula

O muliplex PCR

i/g,m multiplex PCR

r/z10multiplex PCR



O

H1

H2

S. enterica Serovars

Animal Origin (n)1

B

C1

C2

D1

E1

i

g,m

r

z10

1,2

e,n,x


A

a

1,5

Paratyphi A

1 (1)

0

0

0

1

0

0

0

0

0

1

0

B

b

1,2

Paratyphi B

1 (1)

1

0

0

0

0

0

0

0

0

1

0

B

e,h

1,2

Saintpaul

1(1)

1

0

0

0

0

0

0

0

0

1

0

B

e,h

1,5

Reading

1(2)

2

0

0

0

0

0

0

0

0

1

0

B

f,g

-

Derby

1(1)

1

0

0

0

0

0

1

0

0

0

0

B

i

1,2

Typhimurium

1, 4–6 (74)

74

0

0

0

0

74

0

0

0

74

0

B

l,v

1,7

Bredeney

1(1)

1

0

0

0

0

0

0

0

0

1

0

B

1,v

e,n,z15

Brandenburg

1(2)

2

0

0

0

0

0

0

0

0

0

2

B

b

-

Java

6(1)

1

0

0

0

0

0

0

0

0

0

0

B

e,h

e,n,x

Chester

1(2)

2

0

0

0

0

0

0

0

0

0

2

B

f,g,s

-

Agona

1 (1)

1

0

0

0

0

0

1

0

0

0

0

B

r

1,2

Heidelberg

1, 3–6(24)

24

0

0

0

0

0

0

24

0

24

0

B

z

1,5

Kiambu

1(1)

1

0

0

0

0

0

0

0

0

1

0

B

z

1,7

Indiana

1(2)

2

0

0

0

0

0

0

0

0

2

0

B

z10

1,2

Haifa

6 (1)

1

0

0

0

0

0

0

0

1

1

0

C1

b

l,w

Ohio

1(1)

0

1

0

0

0

0

0

0

0

0

0

C1

c

1,5

Choleraesuis

1, 6(6)

0

6

0

0

0

0

0

0

0

6

0

C1

c

1,5

Paratyphi C

1 (1)

0

1

0

0

0

0

0

0

0

1

0

C1

d

l,w

Livingstone

6(1)

0

1

0

0

0

0

0

0

0

0

0

C1

g,m,s

-

Montevideo

1, 5(12)

0

12

0

0

0

0

12

0

0

0

0

C1

k

1,5

Thompson

1(1)

0

1

0

0

0

0

0

0

0

1

0

C1

m,t

-

Oranienburg

1(1)

0

1

0

0

0

0

1

0

0

0

0

C1

z29

-

Tennessee

1(1)

0

1

0

0

0

0

0

0

0

0

0

C1

e,h

e,n,z15

Braenderup

1(2)

0

2

0

0

0

0

0

0

0

0

2

C1

r

1,5

Infantis

1(2)

0

2

0

0

0

0

0

2

0

2

0

C1

z10

e,n,z15

Mbandaka

1(14)

0

14

0

0

0

0

0

0

14

0

14

C1

z28

-

Lille

1(1)

0

1

0

0

0

0

0

0

0

0

0

C2

d

1,2

Muenchen

5(3)

0

0

3

0

0

0

0

0

0

3

0

C2

e,h

1,2

Newport

4,5(1)

0

0

1

0

0

0

0

0

0

1

0

C2

i

z6

Kentucky

1(24)

0

0

24

0

0

24

0

0

0

0

0

C2

z10

e,n,x

Hadar

1 (10)

0

0

10

0

0

0

0

0

10

0

10

D1

a

1,5

Miami

5(1)

0

0

0

1

0

0

0

0

0

1

0

D1

a

1,5

Sendai

5(1)

0

0

0

1

0

0

0

0

0

1

0

D1

g,m

-

Enteritidis

1(20)

0

0

0

20

0

0

20

0

0

0

0

D1

g,p

-

Dublin

2, 6(3)

0

0

0

3

0

0

3

0

0

0

0

D1

l,v

1,5

Panama

1 (1)

0

0

0

1

0

0

0

0

0

1

0

D1

-

-

Gallinarum

1(4)

0

0

0

4

0

0

4

0

0

0

0

D1

f,g,t

-

Berta

1 (2)

0

0

0

2

0

0

2

0

0

0

0

D1

l,z28

1,5

Javiana

1 (1)

0

0

0

1

0

0

0

0

0

1

0

E1

e,h

1,5

Muenster

1(2)

0

0

0

0

2

0

0

0

0

2

0

E1

l,v

1,7

Give

1(2)

0

0

0

0

2

0

0

0

0

2

0

E1

e,h

1,6

Anatum

1, 5 (4)

0

0

0

0

4

0

0

0

0

4

0

E1

l,v

1,6

London

1(2)

0

0

0

0

2

0

0

0

0

2

0

Total

239

114

43

38

34

10

98

44

26

25

135

30

False Positives

0

0

0

1

0

0

24

0

0

30

18

False Negatives

0

0

0

0

0

0

0

0

0

0

0

Kappa2

1.0

1.0

1.0

0.98

1.0

1.0

0.58

1.0

1.0

0.75

0.54


11 = poultry; 2 = bovine; 3 = swine, 4 = other (includes dog, heron, horse, opossum, parrot, rabbit, and snake); 5 = human; and 6 = unknown. Numbers in parentheses indicate the numbers of isolates for each serovar.

2Agreement between PCR allelotyping and conventional serotyping results

Hong et al. BMC Microbiology 2008 8:178   doi:10.1186/1471-2180-8-178

Open Data