Table 2

Degradation of S. pneumoniae Biofilms with DNase treatment.

Strain

Pulmozyme concentration

[% Reduction]


0 (μg ml-1)

1 (μg ml-1)

100 (μg ml-1)

1000 (μg ml-1)

Biomass (μm3/μm2)

Low BFI

BS68 (9V)

0.20 (0.024)

0.08 (0.005)**

0.04 (0.003)**

0.01 (0.001)**

[95.0]

BS71 (3)

0.11(0.013)

0.03 (0.004)**

0.022 (0.005)**

0.02 (0.007)**

[81.8]

BS73 (6A)

0.51(0.065)

0.19 (0.019)**

0.08 (0.008)**

0.04 (0.003)**

[92.2]

High BFI

BS69 (14)

0.59 (0.126)

0.26 (0.039)*

0.19 (0.020)**

0.14 (0.021)**

[76.3]

BS72 (23F)

2.97 (0.278)

1.50 (0.190)**

0.63 (0.083)**

0.24 (0.027)**

[92.0]

BS75 (19F)

0.42 (0.041)

0.38 (0.028)

0.18 (0.020) **

0.14 (0.017)**

[66.7]

Average thickness (μm)

Low BFI

BS68 (9V)

0.23 (0.032)

0.08 (0.006)**

0.03 (0.003)**

0.01 (0.002)**

[95.7]

BS71 (3)

0.19 (0.048)

0.03 (0.017)**

0.003 (0.000)**

0.004 (0.001)**

[94.7]

BS73 (6A)

0.46 (0.068)

0.16 (0.020)**

0.04 (0.005)**

0.02 (0.002)**

[95.7]

High BFI

BS 69 (14)

0.46 (0.068)

0.16 (0.020)**

0.04 (0.005)**

0.02 (0.002)**

[91.3]

BS72 (23F)

5.23 (0.567)

2.23 (0.404)**

0.43 (0.073)**

0.15 (0.027)**

[97.1]

BS75 (19F)

0.39 (0.039)

0.26 (0.024)**

0.12 (0.017)**

0.058 (0.006)**

[85.1]

Maximum thickness (μm)

Low BFI

BS68 (9V)

3.6 (0.147)

3.23 (0.091)

2.44 (0.112)**

2.72 (0.275)**

[24.4]

BS71 (3)

5.2 (0.169)

3.72 (0.219)**

2.32 (0.099)**

2.00 (0.148)**

[61.5]

BS73 (6A)

5.28 (0.249)

3.2 (0.082)**

2.56 (0.110)**

2.56 (0.073)**

[51.5]

High BFI

BS 69 (14)

7.12 (0.301)

4.72 (0.343)**

4.24 (0.193)**

3.6 (0.159)**

[49.4]

BS72 (23F)

10.08 (0.653)

4.96 (0.331)**

3.36 (0.160)**

3.68 (0.309)**

[63.5]

BS75 (19F)

4.20 (0.265)

3.84 (0.198)

3.04 (0.110)**

2.24 (0.110)**

[44.3]


Value = average (n = 10)

Percent reduction of biofilm with highest dose of Pulmozyme.

(Standard error) * p < 0.05; ** p < 0.01

Hall-Stoodley et al. BMC Microbiology 2008 8:173   doi:10.1186/1471-2180-8-173

Open Data