CLSM images of biofilm development by clinical isolates of S. pneumoniae stained with BacLight after 6 days of culture showing viable (green fluorescence) and nonviable (red fluorescence) pneumococci within the biofilms. Images are maximum projections or reconstructed confocal stacks consisting of a series of x-y sections. Sideviews (YZ – left and XZ – bottom) are saggital sections of the biofilm. Scale bar = 30 μm.
Hall-Stoodley et al. BMC Microbiology 2008 8:173 doi:10.1186/1471-2180-8-173