Table 1

Salmonella strains used in the selectivity testing of real-time multiplex PCR assay and traditional cultural method. CT values are given in parentheses.
Salmonella serotypes
 ISO 6579
 Real-time multiplex PCR


aceK
 fliC
 sefA
 sdf
Agona
 +a
 + (23.31)
 -b
 -
 -
Anatum
 +
 + (21.67)
 -
 -
 -
Braenderup
 +
 + (21.24)
 -
 -
 -
Bredeney
 +
 + (21.71)
 -
 -
 -
Derby
 +
 + (22.79)
 -
 -
 -
Dublin (n = 7)c
 +
 + (17.63)
 -
 + (19.35)
 -
Enteritidis (n = 11)
 +
 + (21.35)
 -
 + (23.36)
 + (23.67)
Gallinarum (n = 3)
 +
 + (26.87)
 -
 + (27.83)
 -
Goldcoast
 +
 + (24.35)
 -
 -
 -
Hadar
 +
 + (19.68)
 -
 -
 -
Heidelberg
 +
 + (21.12)
 -
 -
 -
Infantis
 +
 + (23.34)
 -
 -
 -
Kentucky (n = 8)
 +
 + (18.59)
 + (21.65)
 -
 -
Livingstone
 +
 + (21.90)
 -
 -
 -
London
 +
 + (19.92)
 -
 -
 -
Manhattan
 +
 + (17.53)
 -
 -
 -
Newport
 +
 + (18.04)
 -
 -
 -
Nottingham
 +
 + (21.89)
 -
 -
 -
Panama
 +
 + (19.54)
 -
 -
 -
Saintpaul
 +
 + (21.06)
 -
 -
 -
Senftenberg
 +
 + (21.01)
 -
 -
 -
Stanley
 +
 + (19.23)
 -
 -
 -
Typhimurium (n = 10)
 +
 + (19.77)
 + (22.45)
 -
 -
Uganda
 +
 + (20.21)
 -
 -
 -
Virchow
 +
 + (22.52)
 -
 -
 -
Gaminara
 +
 + (21.03)
 -
 -
 -

a + = Salmonella positive by the method.

b – = Salmonella negative by the method.

c = Mean CT values are given when n > 1.

O'Regan et al. BMC Microbiology 2008 8:156   doi:10.1186/1471-2180-8-156